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Journal of Biomedical Optics • Open Access

Repeated freeze-thawing of bone tissue affects Raman bone quality measurements
Author(s): John-David P. McElderry; Matthew R. Kole; Michael D. Morris

Paper Abstract

The ability to probe fresh tissue is a key feature to biomedical Raman spectroscopy. However, it is unclear how Raman spectra of calcified tissues are affected by freezing. In this study, six transverse sections of femoral cortical bone were subjected to multiple freeze/thaw cycles and probed using a custom Raman microscope. Significant decreases were observed in the amide I and amide III bands starting after two freeze thaw cycles. Raman band intensities arising from proline residues of frozen tissue appeared consistent with fresh tissue after four cycles. Crystallinity values of bone mineral diminished slightly with freezing and were noticeable after only one freezing. Mineral carbonate levels did not deviate significantly with freezing and thawing. The authors recommend freezing and thawing bone tissue only once to maintain accurate results.

Paper Details

Date Published: 1 July 2011
PDF: 5 pages
J. Biomed. Opt. 16(7) 071407 doi: 10.1117/1.3574525
Published in: Journal of Biomedical Optics Volume 16, Issue 7
Show Author Affiliations
John-David P. McElderry, Univ. of Michigan (United States)
Matthew R. Kole, Univ. of Michigan (United States)
Michael D. Morris, Univ. of Michigan (United States)

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