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Journal of Biomedical Optics

Multiphoton microscopy study of the morphological and quantity changes of collagen and elastic fiber components in keloid disease
Author(s): Jianxin Chen; Shuangmu Zhuo; Xingshan Jiang; Xiaoqin Zhu; Liqin Zheng; Shusen Xie; Bifang Lin; Haishan Zeng
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Paper Abstract

Multiphoton microscopy was used to study the extracellular matrix of keloid at the molecular level without tissue fixation and staining. Direct imaging of collagen and elastin was achieved by second harmonic generation and two-photon excited fluorescence, respectively. The morphology and quantity of collagen and elastin in keloid were characterized and quantitatively analyzed in comparison to normal skin. The study demonstrated that in keloid, collagen content increased in both the upper dermis and the deep dermis, while elastin mostly showed up in the deep dermis and its quantity is higher compared to normal skin. This suggests the possibility that abnormal fibroblasts synthesized an excessive amount of collagen and elastin at the beginning of keloid formation, corresponding to the observed deep dermis, while after a certain time point, the abnormal fibroblast produced mostly collagen, corresponding to the observed upper dermis. The morphology of collagen and elastin in keloid was disrupted and presented different variations. In the deep dermis, elastic fibers showed node structure, while collagen showed obviously regular gaps between adjacent bundles. In the upper dermis, collagen bundles aligned in a preferred direction, while elastin showed as sparse irregular granules. This new molecular information provided fresh insight about the development process of keloid.

Paper Details

Date Published: 1 May 2011
PDF: 7 pages
J. Biomed. Opt. 16(5) 051305 doi: 10.1117/1.3569617
Published in: Journal of Biomedical Optics Volume 16, Issue 5
Show Author Affiliations
Jianxin Chen, Fujian Normal Univ. (China)
Shuangmu Zhuo, Fujian Normal Univ. (China)
Xingshan Jiang, Fujian Normal Univ. (China)
Xiaoqin Zhu, Fujian Normal Univ. (China)
Liqin Zheng, Fujian Normal Univ. (China)
Shusen Xie, Fujian Normal Univ. (China)
Bifang Lin, Fuzhou First Hospital (China)
Haishan Zeng, The BC Cancer Agency Research Ctr. (Canada)

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