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Journal of Biomedical Optics

Cell tracking in live Caenorhabditis elegans embryos via third harmonic generation imaging microscopy measurements
Author(s): George J. Tserevelakis; George Filippidis; Costas Fotakis; Evgenia V. Megalou; Nektarios Tavemarakis
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Paper Abstract

In this study, we demonstrate the potential of employing third harmonic generation (THG) imaging microscopy measurements for cell tracking studies in live Caenorhabditis elegans (C. elegans) embryos. A 1028-nm femtosecond laser was used for the excitation of unstained C. elegans samples. Different C. elegans embryonic stages (from two-cell to threefold) were imaged. Live biological specimens were irradiated for prolonged periods of time (up to 7 h), testifying to the nondestructive nature of this nonlinear imaging technique. Thus, THG image contrast modality is a powerful diagnostic tool for probing in vivo cell division during early embryogenesis.

Paper Details

Date Published: 1 April 2011
PDF: 7 pages
J. Biomed. Opt. 16(4) 046019 doi: 10.1117/1.3569615
Published in: Journal of Biomedical Optics Volume 16, Issue 4
Show Author Affiliations
George J. Tserevelakis, Univ. of Crete (Greece)
George Filippidis, Foundation for Research and Technology-Hellas (Greece)
Costas Fotakis, Foundation for Research and Technology-Hellas (Greece)
Evgenia V. Megalou, Foundation for Research and Technology-Hellas (Greece)
Nektarios Tavemarakis, Foundation for Research and Technology-Hellas (Greece)


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