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Journal of Biomedical Optics

Long-term imaging of mouse embryos using adaptive harmonic generation microscopy
Author(s): Anisha Thayil; Alexander Jesacher; Tony Wilson; Martin Booth; Tomoko Watanabe; Shankar Srinivas
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Paper Abstract

We present a detailed description of an adaptive harmonic generation (HG) microscope and culture techniques that permit long-term, three-dimensional imaging of mouse embryos. HG signal from both pre- and postimplantation stage (0.5-5.5 day-old) mouse embryos are fully characterized. The second HG images reveal central spindles during cytokinesis whereas third HG images show several features, such as lipid droplets, nucleoli, and plasma membranes. The embryos are found to develop normally during one-day-long discontinuous HG imaging, permitting the observation of several dynamic events, such as morula compaction and blastocyst formation.

Paper Details

Date Published: 1 April 2011
PDF: 9 pages
J. Biomed. Opt. 16(4) 046018 doi: 10.1117/1.3569614
Published in: Journal of Biomedical Optics Volume 16, Issue 4
Show Author Affiliations
Anisha Thayil, Univ. of Oxford (United Kingdom)
Alexander Jesacher, Univ. of Oxford (United Kingdom)
Tony Wilson, Univ. of Oxford (United Kingdom)
Martin Booth, Univ. of Oxford (United Kingdom)
Tomoko Watanabe, Univ. of Oxford (United Kingdom)
Shankar Srinivas, Univ. of Oxford (United Kingdom)

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