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Journal of Biomedical Optics

Waveguide evanescent field fluorescence microscopy: high contrast imaging of a domain forming mixed lipid Langmuir-Blodgett monolayer mimicking lung surfactant
Author(s): Abdollah Hassanzadeh; Silvia Mittler
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Paper Abstract

Waveguide evanescent field fluorescence (WEFF) microscopy is a new development that allows the imaging of contact regions between biological cells and their substratum, as well as imaging of ultrathin films such as monomolecular Langmuir-Blodgett (LB) films. Mixed-lipid monolayer systems mimicking lung surfactant were fabricated on waveguides using the LB technique and imaged by both WEFF and standard wide field epi-fluorescence microscopy. These two technologies were compared with respect to contrast, photobleaching, and sensitivity. It was found that WEFF microscopy produced images with a much higher contrast, lower photobleaching, and higher sensitivity. In addition, fine structures in the lipidic domains were observed for the first time.

Paper Details

Date Published: 1 April 2011
PDF: 5 pages
J. Biomed. Opt. 16(4) 046022 doi: 10.1117/1.3569095
Published in: Journal of Biomedical Optics Volume 16, Issue 4
Show Author Affiliations
Abdollah Hassanzadeh, The Univ. of Western Ontario (Canada)
Silvia Mittler, The Univ. of Western Ontario (Canada)

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