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Journal of Biomedical Optics

Effect of 405-nm high-intensity narrow-spectrum light on fibroblast-populated collagen lattices: an in vitro model of wound healing
Author(s): Richard McDonald; M. Helen Grant; Scott J. MacGregor; John G. Anderson; Michelle Maclean
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Paper Abstract

High-intensity narrow-spectrum (HINS) 405-nm light is a novel technology developed to address the significant problem of health-care associated infection. Its potential for wound-decontamination applications is assessed on mammalian cells and bacteria. The fibroblast-populated collagen lattice (FPCL) is used as an in vitro model of wound healing, and the effect of HINS light on contraction is examined. Effects on cell proliferation, morphological changes, and α-smooth muscle actin (α-SMA) expression are investigated. Bactericidal effects are assessed using the bacterium Staphylococcus epidermidis. Low doses of HINS light were found to have no significant inhibitory effects on FPCL contraction, cell proliferation, or α-SMA expression. Doses of up to 18 Jcm−2 had no significant inhibitory effects on FPCL cell numbers, and this dose was shown to cause almost complete inactivation of bacteria. These results show that HINS light has potential for disinfection applications without adversely influencing wound healing.

Paper Details

Date Published: 1 April 2011
PDF: 5 pages
J. Biomed. Opt. 16(4) 048003 doi: 10.1117/1.3561903
Published in: Journal of Biomedical Optics Volume 16, Issue 4
Show Author Affiliations
Richard McDonald, Univ. of Strathclyde (United Kingdom)
M. Helen Grant, Univ. of Strathclyde (United Kingdom)
Scott J. MacGregor, Univ. of Strathclyde (United Kingdom)
John G. Anderson, Univ. of Strathclyde (United Kingdom)
Michelle Maclean, Univ. of Strathclyde (United Kingdom)


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