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Journal of Biomedical Optics • Open Access

Development of a stigmatic mass microscope using laser desorption/ionization and a multi-turn time-of-flight mass spectrometer
Author(s): Hisanao Hazama; Hidetoshi Yoshimura; Kunio Awazu; Toshio Tashima; Jun Aoki; Michisato Toyoda; Hirofumi Nagao; Katsuyoshi Masuda; Kenichi Fujii; Yasuhide Naito

Paper Abstract

A novel stigmatic mass microscope using laser desorption/ionization and a multi-turn time-of-flight mass spectrometer, MULTUM-IMG, has been developed. Stigmatic ion images of crystal violet masked by a fine square mesh grid with a 12.7 μm pitch as well as microdot patterns with a 5 μm dot diameter and a 10 μm pitch made with rhodamine B were clearly observed. The estimated spatial resolution was about 3 μm in the linear mode with a 20-fold ion optical magnification. Separating stigmatic ion images according to the time-of-flight, i.e., the mass-to-charge ratio of the ions was successfully demonstrated by a microdot pattern made with two different dyes, crystal violet and methylene blue. Stigmatic ion images of a microdot pattern made with crystal violet were observed after circulation in MULTUM-IMG, and the pattern of the ion image was maintained after ten cycles in MULTUM-IMG. A section of a mouse brain stained with crystal violet and methylene blue was observed in the linear mode, and the stigmatic total ion image of crystal violet and methylene blue agreed well with the optical microphotograph of the hippocampus for the same section.

Paper Details

Date Published: 1 April 2011
PDF: 8 pages
J. Biomed. Opt. 16(4) 046007 doi: 10.1117/1.3561091
Published in: Journal of Biomedical Optics Volume 16, Issue 4
Show Author Affiliations
Hisanao Hazama, Osaka Univ. (Japan)
Hidetoshi Yoshimura, Osaka Univ. (Japan)
Kunio Awazu, Osaka Univ. (Japan)
Toshio Tashima, Japan Science and Technology Agency (Japan)
Jun Aoki, Osaka Univ. (Japan)
Michisato Toyoda, Osaka Univ. (Japan)
Hirofumi Nagao, Osaka Univ. (Japan)
Katsuyoshi Masuda, Suntory Institute for Bioorganic Research (Japan)
Kenichi Fujii, Osaka Institute of Technology (Japan)
Yasuhide Naito, The Graduate School for the Creation of New Photonics Industries (Japan)


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