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Journal of Biomedical Optics

Reconstruction of fluorescence distribution hidden in biological tissue using mesoscopic epifluorescence tomography
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Paper Abstract

Mesoscopic epifluorescence tomography is a novel technique that discovers fluorescence bio-distribution in small animals by tomographic means in reflectance geometry. A collimated laser beam is scanned over the skin surface to excite fluorophores hidden within the tissue while a CCD camera acquires an image of the fluorescence emission for each source position. This configuration is highly efficient in the visible spectrum range where trans-illumination imaging of small animals is not feasible due to the high tissue absorption and scattering in biological organisms. The reconstruction algorithm is similar to the one used in fluorescence molecular tomography. However, diffusion theory cannot be employed since the source-detector separation for most image pixels is comparable to or below the scattering length of the tissue. Instead Monte Carlo simulations are utilized to predict the sensitivity functions. In a phantom study we show the effect of using enhanced source grid arrangements during the data acquisition and the reconstruction process to minimize boundary artefacts. Furthermore, we present ex vivo data that show high spatial resolution and quantitative accuracy in heterogeneous tissues using GFP-like fluorescence in B6-albino mice up to a depth of 1100 μm.

Paper Details

Date Published: 1 April 2011
PDF: 9 pages
J. Biomed. Opt. 16(4) 046005 doi: 10.1117/1.3560631
Published in: Journal of Biomedical Optics Volume 16, Issue 4
Show Author Affiliations
Saskia Bjorn, Helmholtz Zentrum München GmbH (Germany)
Karl-Hans Englmeier, Helmholtz Zentrum München GmbH (Germany)
Vasilis Ntziachristos, Helmholtz Zentrum München GmbH (Germany)
Ralf B. Schulz, Helmholtz Zentrum München GmbH (Germany)

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