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Journal of Biomedical Optics • Open Access

Intravital microscopy of subpleural alveoli via transthoracic endoscopy
Author(s): David Schwenninger; Hanna Runck; Stefan Schumann; Josef Guttmann; Jorg Haberstroh; Sven Meissner; Edmund Koch

Paper Abstract

Transfer of too high mechanical energy from the ventilator to the lung's alveolar tissue is the main cause for ventilator-induced lung injury (VILI). To investigate the effects of cyclic energy transfer to the alveoli, we introduce a new method of transthoracic endoscopy that provides morphological as well as functional information about alveolar geometry and mechanics. We evaluate the new endoscopic method to continuously record images of focused subpleural alveoli. The method is evaluated by using finite element modeling techniques and by direct observation of subpleural alveoli both in isolated rat lungs as well as in intact animals (rats). The results confirm the overall low invasiveness of the endoscopic method insofar as the mechanical influences on the recorded alveoli are only marginal. It is, hence, a suited method for intravital microscopy in the rat model as well as in larger animals.

Paper Details

Date Published: 1 April 2011
PDF: 6 pages
J. Biomed. Opt. 16(4) 046002 doi: 10.1117/1.3560297
Published in: Journal of Biomedical Optics Volume 16, Issue 4
Show Author Affiliations
David Schwenninger, Univ. Hospital Freiburg (Germany)
Hanna Runck, Univ. Hospital Freiburg (Germany)
Stefan Schumann, Univ. Hospital Freiburg (Germany)
Josef Guttmann, Univ. Hospital Freiburg (Germany)
Jorg Haberstroh, Univ. Hospital Freiburg (Germany)
Sven Meissner, Universitätsklinikum Carl Gustav Carus Dresden (Germany)
Edmund Koch, Universitätsklinikum Carl Gustav Carus Dresden (Germany)

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