Share Email Print
cover

Journal of Biomedical Optics

Kinetics of carotenoid distribution in human skin in vivo after exogenous stress: disinfectant and wIRA-induced carotenoid depletion recovers from outside to inside
Author(s): Joachim W. Fluhr; Heike Richter; Wolfram Sterry; Jürgen M. Lademann; Maxim E. Darvin; Peter J. Caspers; J. Andre van der Pol
Format Member Price Non-Member Price
PDF $20.00 $25.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

The human organism has developed a protection system against the destructive effect of free radicals. The aim of the present study was to investigate the extent of exogenous stress factors such as disinfectant and IR-A radiation on the skin, and their influence on the kinetics of carotenoids distribution during the recovery process. Ten healthy volunteers were assessed with resonance spectroscopy using an Argon-laser at 488 nm to excite the carotenoids in vivo. Additionally, Raman-confocal-micro-spectroscopy measurements were performed using a model 3510 Skin Composition Analyzer with spatially resolved measurements down to 30 μm. The measurements were performed at a baseline of 20, 40, 60, and 120 min after an external stressor consisting either of water-filtered infrared A (wIRA) with 150 mW/cm2 or 1 ml/cm2 of an alcoholic disinfectant. Both Raman methods were capable to detect the infrared-induced depletion of carotenoids. Only Raman-microspectroscopy could reveal the carotenoids decrease after topical disinfectant application. The carotenoid-depletion started at the surface. After 60 min, recovery starts at the surface while deeper parts were still depleted. The disinfectant- and wIRA-induced carotenoid depletion in the epidermis recovers from outside to inside and probably delivered by sweat and sebaceous glands. We could show that the Raman microscopic spectroscopy is suited to analyze the carotenoid kinetic of stress effects and recovery.

Paper Details

Date Published: 1 March 2011
PDF: 8 pages
J. Biomed. Opt. 16(3) 035002 doi: 10.1117/1.3555183
Published in: Journal of Biomedical Optics Volume 16, Issue 3
Show Author Affiliations
Joachim W. Fluhr, Charité Universitätsmedizin Berlin (Germany)
Heike Richter, Charité Universitätsmedizin Berlin (Germany)
Wolfram Sterry, Charité Universitätsmedizin Berlin (Germany)
Jürgen M. Lademann, Charité Universitätsmedizin Berlin (Germany)
Maxim E. Darvin, Charité Universitätsmedizin Berlin (Germany)
Peter J. Caspers, River Diagnostics B.V. (Netherlands)
J. Andre van der Pol, River Diagnostics B.V. (Netherlands)


© SPIE. Terms of Use
Back to Top