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Journal of Biomedical Optics • Open Access

Label-free intracellular transport measured by spatial light interference microscopy
Author(s): Zhuo Wang; Huafeng Ding; Rashid Bashir; Gabriel Popescu; Larry Millet; Martha Gillette; Vincent Chan

Paper Abstract

We show that applying the Laplace operator to a speckle-free quantitative phase image reveals an unprecedented level of detail in cell structure, without the gradient artifacts associated with differential interference contrast microscopy, or photobleaching and phototoxicity limitations common in fluorescence microscopy. This method, referred to as Laplace phase microscopy, is an efficient tool for tracking vesicles and organelles in living cells. The principle is demonstrated by tracking organelles in cardiomyocytes and vesicles in neurites of hippocampal neurons, which to our knowledge are the first label-free diffusion measurements of the organelles in such cells.

Paper Details

Date Published: 1 February 2011
PDF: 9 pages
J. Biomed. Opt. 16(2) 026019 doi: 10.1117/1.3549204
Published in: Journal of Biomedical Optics Volume 16, Issue 2
Show Author Affiliations
Zhuo Wang, Univ. of Illinois at Urbana-Champaign (United States)
Huafeng Ding, Univ. of Illinois at Urbana-Champaign (United States)
Rashid Bashir, Univ. of Illinois at Urbana-Champaign (United States)
Gabriel Popescu, Univ. of Illinois at Urbana-Champaign (United States)
Larry Millet, Univ. of Illinois at Urbana-Champaign (United States)
Martha Gillette, Univ. of Illinois at Urbana-Champaign (United States)
Vincent Chan, Univ. of Illinois at Urbana-Champaign (United States)


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