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Journal of Biomedical Optics

Dynamic analysis of pathogen-infected host cells using quantitative phase microscopy
Author(s): Seungrag Lee; Ji Yong Lee; Chang-Soo Park; Young Ran Kim; Joon Haeng Rhee; Dug Young Kim
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Paper Abstract

We present the real-time quantitative analysis of Vibrio vulnificus-infected host cells using quantitative phase microscopy (QPM) based on interferometric techniques. This provides the ability to retrieve the phase or optical path-length distribution over the cell with nanometer path-length sensitivity from a single interferogram image. We have used QPM to study dynamic cell morphologic changes and to noninvasively quantify the cell volumes of rat basophilic leukemia RBL-2H3 cells infected with V. vulnificus strains: wild type (MO6-24/O) and RtxA1 toxin mutant (CMM770). During the process of V. vulnificus infection in RBL-2H3 cells, the dynamic changes of quantitative phase images, cell volumes, and areas were observed in real time using QPM. In contrast, dramatic changes were not detected in RBL-2H3 cells infected with the noncytotoxic RtxA1 toxin mutant. The results showed good correlation between QPM analysis and biochemical assays, such as lactate dehydrogenase assay or β-hexosaminidase release assay. We suggest that QPM is a powerful quantitative method to study the dynamic process of host cells infected with pathogens in a noninvasive manner.

Paper Details

Date Published: 1 March 2011
PDF: 8 pages
J. Biomed. Opt. 16(3) 036004 doi: 10.1117/1.3548882
Published in: Journal of Biomedical Optics Volume 16, Issue 3
Show Author Affiliations
Seungrag Lee, Gwangju Institute of Science and Technology (Korea, Republic of)
Ji Yong Lee, Gwangju Institute of Science and Technology (Korea, Republic of)
Chang-Soo Park, Gwangju Institute of Science and Technology (Korea, Republic of)
Young Ran Kim, Dongshin Univ. (Korea, Republic of)
Joon Haeng Rhee, Chonnam National Univ. Medical School (Korea, Republic of)
Dug Young Kim, Yonsei Univ. (Korea, Republic of)

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