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Journal of Biomedical Optics

Deep in vivo two-photon imaging of blood vessels with a new dye encapsulated in pluronic nanomicelles
Author(s): Mathieu Maurin; Olivier Stephan; Jean-Claude A. Vial; Seth R. Marder; Boudewijn P. J. van der Sanden
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Paper Abstract

Our purpose is to test if Pluronic® fluorescent nanomicelles can be used for in vivo two-photon imaging of both the normal and the tumor vasculature. The nanomicelles were obtained after encapsulating a hydrophobic two-photon dye: di-stryl benzene derivative, in Pluronic block copolymers. Their performance with respect to imaging depth, blood plasma staining, and diffusion across the tumor vascular endothelium is compared to a classic blood pool dye Rhodamin B dextran (70 kDa) using two-photon microscopy. Pluronic nanomicelles show, like Rhodamin B dextran, a homogeneous blood plasma staining for at least 1 h after intravenous injection. Their two-photon imaging depth is similar in normal mouse brain, using 10 times less injected mass. In contrast with Rhodamin B dextran, no extravasation is observed in leaky tumor vessels due to their large size: 20-100 nm. In conclusion, Pluronic nanomicelles can be used as a blood pool dye, even in leaky tumor vessels. The use of Pluronic block copolymers is a valuable approach for encapsulating two-photon fluorescent dyes that are hydrophobic and not suitable for intravenous injection.

Paper Details

Date Published: 1 March 2011
PDF: 7 pages
J. Biomed. Opt. 16(3) 036001 doi: 10.1117/1.3548879
Published in: Journal of Biomedical Optics Volume 16, Issue 3
Show Author Affiliations
Mathieu Maurin, Univ. Joseph Fourier (France)
Olivier Stephan, Univ. Joseph Fourier (France)
Jean-Claude A. Vial, Univ. Joseph Fourier (France)
Seth R. Marder, Georgia Institute of Technology (United States)
Boudewijn P. J. van der Sanden, Institut National de la Santé et de la Recherche Médicale (France)

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