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Journal of Biomedical Optics • Open Access

Drug-loaded biodegradable microspheres for image-guided combinatory epigenetic therapy in cells
Author(s): Ronald X. Xu; Songbo Xu; Tao Zuo; Tim H. Huang; Rulong Shen; Michael F. Tweedle

Paper Abstract

We synthesize drug-loaded poly (lactic-co-glycolic acid) (PLGA) microspheres for image-guided combinatory epigenetic therapy in MCF-10A human mammary epithelial cells. LY294002 and Nile Red are encapsulated in microspheres for sustained drug release and fluorescence microscopic imaging. Drug-loaded microspheres target MCF-10A cells through a three-step binding process involving biotinylated antibody, streptavidin, and biotinylated microspheres. LY294002 loaded microspheres and 5-Aza-2-deoxycytidine are applied to MCF-10A cells for combinatory PI3K/AKT inhibition and deoxyribonucleic acid (DNA) demethylation. Our study implies the technical potential of disease targeting and image-guided combinatory epigenetic therapy using drug-loaded multifunctional biodegradable PLGA microspheres.

Paper Details

Date Published: 1 February 2011
PDF: 3 pages
J. Biomed. Opt. 16(2) 020507 doi: 10.1117/1.3548878
Published in: Journal of Biomedical Optics Volume 16, Issue 2
Show Author Affiliations
Ronald X. Xu, The Ohio State Univ. (United States)
Songbo Xu, The Ohio State Univ. (United States)
Tao Zuo, The Ohio State Univ. (United States)
Tim H. Huang, The Ohio State Univ. (United States)
Rulong Shen, The Ohio State Univ. (United States)
Michael F. Tweedle, The Ohio State Univ. (United States)


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