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Journal of Biomedical Optics • Open Access

Light-scattering signal may indicate critical time zone to rescue brain tissue after hypoxia
Author(s): Satoko Kawauchi; Miya Ishihara; Makoto Kikuchi; Shunichi Sato; Yoichi Uozumi; Hiroshi Nawashiro; Susanta K. Hui; Nikolaos P. Papanikolopoulos

Paper Abstract

A light-scattering signal, which is sensitive to cellular/subcellular structural integrity, is a potential indicator of brain tissue viability because metabolic energy is used in part to maintain the structure of cells. We previously observed a unique triphasic scattering change (TSC) at a certain time after oxygen/glucose deprivation for blood-free rat brains; TSC almost coincided with the cerebral adenosine triphosphate (ATP) depletion. We examine whether such TSC can be observed in the presence of blood in vivo, for which transcranial diffuse reflectance measurement is performed for rat brains during hypoxia induced by nitrogen gas inhalation. At a certain time after hypoxia, diffuse reflectance intensity in the near-infrared region changes in three phases, which is shown by spectroscopic analysis to be due to scattering change in the tissue. During hypoxia, rats are reoxygenated at various time points. When the oxygen supply is started before TSC, all rats survive, whereas no rats survive when the oxygen supply is started after TSC. Survival is probabilistic when the oxygen supply is started during TSC, indicating that the period of TSC can be regarded as a critical time zone for rescuing the brain. The results demonstrate that light scattering signal can be an indicator of brain tissue reversibility.

Paper Details

Date Published: 1 February 2011
PDF: 8 pages
J. Biomed. Opt. 16(2) 027002 doi: 10.1117/1.3542046
Published in: Journal of Biomedical Optics Volume 16, Issue 2
Show Author Affiliations
Satoko Kawauchi, National Defense Medical College (Japan)
Miya Ishihara, National Defense Medical College (Japan)
Makoto Kikuchi, National Defense Medical College (Japan)
Shunichi Sato, National Defense Medical College (Japan)
Yoichi Uozumi, National Defense Medical College (Japan)
Hiroshi Nawashiro, National Defense Medical College (Japan)
Susanta K. Hui, Univ. of Minnesota, Twin Cities (United States)
Nikolaos P. Papanikolopoulos, Univ. of Minnesota, Twin Cities (United States)

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