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Journal of Biomedical Optics

Hyperspectral coherent anti-Stokes Raman scattering microscopy imaging through turbid medium
Author(s): Rajan Arora; Georgi I. Petrov; Vladislav V. Yakovlev
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Paper Abstract

Coherent Raman microspectroscopy imaging is an emerging technique for noninvasive, chemically specific optical imaging, which can be potentially used to analyze the chemical composition and its distribution in biological tissues. In this report, a hierarchical cluster analysis was applied to hyperspectral coherent anti-Stokes Raman imaging of different chemical species through a turbid medium. It was demonstrated that by using readily available commercial software (Cytospec, Inc.) and cluster analysis, distinct chemical species can be imaged and identified through a rather thick layer of scattering medium. Once the clusters of different chemical composition were distinguished, a phase retrieval algorithm was used to convert coherent anti-Stokes Raman spectra to Raman spectra, which were used for chemical identification of hidden microscopic objects. In particular, applications to remote optical sensing of potential biological threats and to imaging through a layer of skin tissue were successfully demonstrated.

Paper Details

Date Published: 1 February 2011
PDF: 8 pages
J. Biomed. Opt. 16(2) 021116 doi: 10.1117/1.3541796
Published in: Journal of Biomedical Optics Volume 16, Issue 2
Show Author Affiliations
Rajan Arora, Univ. of Wisconsin-Milwaukee (United States)
Georgi I. Petrov, Univ. of Wisconsin-Milwaukee (United States)
Vladislav V. Yakovlev, Univ. of Wisconsin-Milwaukee (United States)

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