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Journal of Biomedical Optics

Optical transfection using an endoscope-like system
Author(s): Nan Ma; Kishan Dholakia; Frank J. Gunn-Moore
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Paper Abstract

Optical transfection is a powerful method for targeted delivery of therapeutic agents to biological cells. A tightly focused pulsed laser beam may transiently change the permeability of a cell membrane to facilitate the delivery of foreign genetic material into cells. We report the first realization of an endoscope-like integrated system for optical transfection. An imaging fiber (coherent optical fiber bundle) with ∼6000 cores (pixels) embedded in a fiber cladding of ∼300 μm in diameter, produces an image circle (area) of ∼270 μm diam. This imaging fiber, with an ordered axicon lens array chemically etched at its exit face, is used for the delivery of a femtosecond laser to the cell membrane for optical transfection along with subcellular resolution imaging. A microcapillary-based microfluidic system for localized drug delivery was also combined in this miniature, flexible system. Using this novel system, a plasmid transfection efficiency up to ∼72% was obtained for CHO-K1 cells. This endoscope-like system opens a range of exciting applications, in particular, in the targeted in vivo optical microsurgery area.

Paper Details

Date Published: 1 February 2011
PDF: 7 pages
J. Biomed. Opt. 16(2) 028002 doi: 10.1117/1.3541781
Published in: Journal of Biomedical Optics Volume 16, Issue 2
Show Author Affiliations
Nan Ma, Univ. of St. Andrews (United Kingdom)
Kishan Dholakia, Univ. of St. Andrews (United Kingdom)
Frank J. Gunn-Moore, Univ. of St. Andrews (United Kingdom)


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