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Journal of Biomedical Optics • Open Access

Simplified approach for quantitative digital holographic phase contrast imaging of living cells
Author(s): Björn Kemper; Angelika Vollmer; Gert von Bally; Christina E. Rommel; Jürgen Schnekenburger

Paper Abstract

Many interferometry-based quantitative phase contrast imaging techniques require a separately generated coherent reference wave. This results in a low phase stability and the demand for a precise adjustment of the intensity ratio between object and reference wave. To overcome these problems, the performance of a Michelson interferometer approach for digital holographic microscopy was analyzed that avoids a separately generated reference wave by superposition of different image areas. It is shown that this simplified arrangement yields improved phase stability. Furthermore, results from time-lapse investigations on living pancreas tumor cells demonstrate the capability of the method for reliable quantitative phase contrast imaging.

Paper Details

Date Published: 1 February 2011
PDF: 4 pages
J. Biomed. Opt. 16(2) 026014 doi: 10.1117/1.3540674
Published in: Journal of Biomedical Optics Volume 16, Issue 2
Show Author Affiliations
Björn Kemper, Ctr. for Biomedical Optics and Photonics (Germany)
Angelika Vollmer, Ctr. for Biomedical Optics and Photonics (Germany)
Gert von Bally, Ctr. for Biomedical Optics and Photonics (Germany)
Christina E. Rommel, Westfälische Wilhelms-Univ. Münster (Germany)
Jürgen Schnekenburger, Westfälische Wilhelms-Univ. Münster (Germany)


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