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Journal of Biomedical Optics

Swept-source optical coherence tomography of lower limb wound healing with histopathological correlation
Author(s): Ananya Barui; Rusha Patra; Raunak K. Das; Santanu Dhara; Pranab K. Dutta; Jyotirmoy Chatterjee; Provas Banerjee
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Paper Abstract

Direct noninvasive visualization of wound bed with depth information is important to understand the tissue repair. We correlate skin swept-source-optical coherence tomography (OCT) with histopathological and immunohistochemical evaluation on traumatic lower limb wounds under honey dressing to compare and assess the tissue repair features acquired noninvasively and invasively. Analysis of optical biopsy identifies an uppermost brighter band for stratum corneum with region specific thickness (p < 0.0001) and gray-level intensity (p < 0.0001) variation. Below the stratum corneum, variation in optical intensities is remarkable in different regions of the wound bed. Correlation between OCT and microscopic observations are explored especially in respect to progressive growth and maturation of the epithelial and subepithelial components. Characteristic transition of uniform hypolucid band in OCT image for depigmented zone to wavy highly lucid band in the pigmented zone could be directly correlated with the microscopic findings. The transformation of prematured epithelium of depigmented area, with low expression of E-cadherin, to matured epithelium with higher E-cadherin expression in pigmented zone, implicated plausible change in their optical properties as depicted in OCT. This correlated evaluation of multimodal images demonstrates applicability of swept-source-OCT in wound research and importance of integrated approach in validation of new technology.

Paper Details

Date Published: 1 February 2011
PDF: 8 pages
J. Biomed. Opt. 16(2) 026010 doi: 10.1117/1.3535593
Published in: Journal of Biomedical Optics Volume 16, Issue 2
Show Author Affiliations
Ananya Barui, Indian Institute of Technology Kharagpur (India)
Rusha Patra, Indian Institute of Technology Kharagpur (India)
Raunak K. Das, Indian Institute of Technology Kharagpur (India)
Santanu Dhara, Indian Institute of Technology Kharagpur (India)
Pranab K. Dutta, Indian Institute of Technology Kharagpur (India)
Jyotirmoy Chatterjee, Indian Institute of Technology Kharagpur (India)
Provas Banerjee, Banerjee's Biomedical Research Foundation (India)

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