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Journal of Biomedical Optics • Open Access

High-resolution resonant and nonresonant fiber-scanning confocal microscope
Author(s): Benno H. W. Hendriks; Walter C. J. Bierhoff; Jeroen J. L. Horikx; Adrien E. Desjardins; Cees A. Hezemans; Gert W. t'Hooft; Gerald W. Lucassen; Nenad Mihajlovic

Paper Abstract

We present a novel, hand-held microscope probe for acquiring confocal images of biological tissue. This probe generates images by scanning a fiber-lens combination with a miniature electromagnetic actuator, which allows it to be operated in resonant and nonresonant scanning modes. In the resonant scanning mode, a circular field of view with a diameter of 190 μm and an angular frequency of 127 Hz can be achieved. In the nonresonant scanning mode, a maximum field of view with a width of 69 μm can be achieved. The measured transverse and axial resolutions are 0.60 and 7.4 μm, respectively. Images of biological tissue acquired in the resonant mode are presented, which demonstrate its potential for real-time tissue differentiation. With an outer diameter of 3 mm, the microscope probe could be utilized to visualize cellular microstructures in vivo across a broad range of minimally-invasive procedures.

Paper Details

Date Published: 1 February 2011
PDF: 8 pages
J. Biomed. Opt. 16(2) 026007 doi: 10.1117/1.3534781
Published in: Journal of Biomedical Optics Volume 16, Issue 2
Show Author Affiliations
Benno H. W. Hendriks, Philips Research Nederland B.V. (Netherlands)
Walter C. J. Bierhoff, Philips Research Nederland B.V. (Netherlands)
Jeroen J. L. Horikx, Philips Research Nederland B.V. (Netherlands)
Adrien E. Desjardins, Philips Research Nederland B.V. (Netherlands)
Cees A. Hezemans, Philips Research Nederland B.V. (Netherlands)
Gert W. t'Hooft, Philips Research Nederland B.V. (Netherlands)
Gerald W. Lucassen, Philips Research Nederland B.V. (Netherlands)
Nenad Mihajlovic, Philips Research Nederland B.V. (Netherlands)


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