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Journal of Biomedical Optics

Picosecond spectral coherent anti-Stokes Raman scattering imaging with principal component analysis of meibomian glands
Author(s): Chia-Yu Lin; Eric O. Potma; Jeffrey L. Suhalim; Chyong Ly Nien; James V. Jester; Miloš D. Miljkovic; Max Diem
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Paper Abstract

The lipid distribution in the mouse meibomian gland was examined with picosecond spectral anti-Stokes Raman scattering (CARS) imaging. Spectral CARS data sets were generated by imaging specific localized regions of the gland within tissue sections at consecutive Raman shifts in the CH2 stretching vibrational range. Spectral differences between the location specific CARS spectra obtained in the lipid-rich regions of the acinus and the central duct were observed, which were confirmed with a Raman microspectroscopic analysis, and attributed to meibum lipid modifications within the gland. A principal component analysis of the spectral data set reveals changes in the CARS spectrum when transitioning from the acini to the central duct. These results demonstrate the utility of picosecond spectral CARS imaging combined with multivariate analysis for assessing differences in the distribution and composition of lipids in tissues.

Paper Details

Date Published: 1 February 2011
PDF: 9 pages
J. Biomed. Opt. 16(2) 021104 doi: 10.1117/1.3533716
Published in: Journal of Biomedical Optics Volume 16, Issue 2
Show Author Affiliations
Chia-Yu Lin, Univ. of California, Irvine (United States)
Eric O. Potma, Univ. of California, Irvine (United States)
Jeffrey L. Suhalim, Univ. of California, Irvine (United States)
Chyong Ly Nien, Univ. of California, Irvine (United States)
James V. Jester, Univ. of California, Irvine (United States)
Miloš D. Miljkovic, Northeastern Univ. (United States)
Max Diem, Northeastern Univ. (United States)

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