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Journal of Biomedical Optics

Video-rate wide-field coherent anti-Stokes Raman scattering microscopy with collinear nonphase-matching illumination
Author(s): Ming Lei; Martin Winterhalder; Romedi Selm; Andreas Zumbusch
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Paper Abstract

A simple scheme for video-rate wide-field coherent anti-Stokes Raman scattering (CARS) microscopy is presented. The method is based on collinear nonphase-matching illumination. The mechanisms leading to CARS signal generation are investigated. We find that refraction-mediated phase-matching is the main effect. Video-rate wide-field CARS microscopy of polystyrene beads and CARS wide-field images of C. elegans embryos are shown, and the capabilities and the limitations of the scheme are discussed.

Paper Details

Date Published: 1 February 2011
PDF: 5 pages
J. Biomed. Opt. 16(2) 021102 doi: 10.1117/1.3533707
Published in: Journal of Biomedical Optics Volume 16, Issue 2
Show Author Affiliations
Ming Lei, Xi'an Institute of Optics & Precision Mechanics (China)
Martin Winterhalder, Univ. Konstanz (Germany)
Romedi Selm, Univ. Konstanz (Germany)
Andreas Zumbusch, Univ. Konstanz (Germany)

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