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Journal of Biomedical Optics • Open Access

Biodistribution and photodynamic effects of polyvinylpyrrolidone-hypericin using multicellular spheroids composed of normal human urothelial and T24 transitional cell carcinoma cells
Author(s): Joachim Vandepitte; Mieke Roelants; Ben Van Cleynenbreugel; Klaudia Hettinger; Evelyne Lerut; Hendrik Van Poppel; Peter A. M. de Witte

Paper Abstract

Polyvinylpyrrolidone (PVP)-hypericin is a potent photosensitizer that is used in the urological clinic to photodiagnose with high-sensitivity nonmuscle invasive bladder cancer (NMIBC). We examined the differential accumulation and therapeutic effects of PVP-hypericin using spheroids composed of a human urothelial cell carcinoma cell line (T24) and normal human urothelial (NHU) cells. The in vitro biodistribution was assessed using fluorescence image analysis of 5-μm cryostat sections of spheroids that were incubated with PVP-hypericin. The results show that PVP-hypericin accumulated to a much higher extent in T24 spheroids as compared to NHU spheroids, thereby reproducing the clinical situation. Subsequently, spheroids were exposed to different PDT regimes with a light dose ranging from 0.3 to 18J/cm2. When using low fluence rates, only minor differences in cell survival were seen between normal and malignant spheroids. High light fluence rates induced a substantial difference in cell survival between the two spheroid types, killing ∼80% of the cells present in the T24 spheroids. It was concluded that further in vivo experiments are required to fully evaluate the potential of PVP-hypericin as a phototherapeutic for NMIBC, focusing on the combination of the compound with methods that enhance the oxygenation of the urothelium.

Paper Details

Date Published: 1 January 2011
PDF: 6 pages
J. Biomed. Opt. 16(1) 018001 doi: 10.1117/1.3533316
Published in: Journal of Biomedical Optics Volume 16, Issue 1
Show Author Affiliations
Joachim Vandepitte, Katholieke Univ. Leuven (Belgium)
Mieke Roelants, Katholieke Univ. Leuven (Belgium)
Ben Van Cleynenbreugel, Katholieke Univ. Leuven (Belgium)
Klaudia Hettinger, Sanochemia Pharmazeutika AG (Austria)
Evelyne Lerut, Katholieke Univ. Leuven (Belgium)
Hendrik Van Poppel, Katholieke Univ. Leuven (Belgium)
Peter A. M. de Witte, Katholieke Univ. Leuven (Belgium)

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