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Journal of Biomedical Optics

Chemical imaging and microspectroscopy with spectral focusing coherent anti-Stokes Raman scattering
Author(s): Bi-Chang Chen; Jiha Sung; Xiaoxi Wu; Sang-Hyun Lim
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Paper Abstract

We demonstrate two different coherent anti-Stokes Raman scattering (CARS) microscopy and microspectroscopy methods based on the spectral focusing mechanism. The first method uses strongly chirped broadband pulses from a single Ti:sapphire laser and generates CARS signals at the fingerprint region. Fast modulation of the time delay between the pump and Stokes laser pulses coupled with lock-in signal detection significantly reduces the nonresonant background and produces Raman-like CARS signals with a spectral resolution of 20 cm−1. The second method generates CARS signals in the CH (carbon-hydrogen) stretching region with IR supercontinuum pulses from a photonic crystal fiber. The spectral resolution of 30 cm−1 is achieved. Maximum entropy method is used to retrieve a Raman-equivalent CARS spectrum from lipid membranes. Chemical imaging and microspectroscopy are demonstrated with various samples.

Paper Details

Date Published: 1 February 2011
PDF: 8 pages
J. Biomed. Opt. 16(2) 021112 doi: 10.1117/1.3533315
Published in: Journal of Biomedical Optics Volume 16, Issue 2
Show Author Affiliations
Bi-Chang Chen, The Univ. of Texas at Austin (United States)
Jiha Sung, The Univ. of Texas at Austin (United States)
Xiaoxi Wu, The Univ. of Texas at Austin (United States)
Sang-Hyun Lim, The Univ. of Texas at Austin (United States)


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