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Journal of Biomedical Optics • Open Access

Real-time imaging of laser-induced membrane disruption of a living cell observed with multifocus coherent anti-Stokes Raman scattering microscopy
Author(s): Takeo Minamikawa; Tsutomu Araki; Mamoru Hashimoto; Hirohiko Niioka

Paper Abstract

We demonstrate the real-time imaging of laser-induced disruption of the cellular membrane in a living HeLa cell and its cellular response with a multifocus coherent anti-Stokes Raman scattering (CARS) microscope. A near-infrared pulsed laser beam tightly focused on the cellular membrane of a living cell induces ablation at the focal point causing a local disruption of the cellular membrane. After the membrane disruption a dark spot decreasing CARS intensity of 2840 cm−1 Raman shift at the disrupted site appears. This dark spot immediately disappears and a strong CARS signal is observed around the disrupted site. This increase of the CARS signal might be caused by resealing of the disrupted site via aggregation of the patch lipid vesicles in the cytoplasm. The accumulation of lipids around the disrupted site is also confirmed with three-dimensional CARS images of a cell before and after membrane disruption. The temporal behavior of the CARS signal at the disrupted site is observed to detect the fusion dynamics of patch vesicles

Paper Details

Date Published: 1 February 2011
PDF: 5 pages
J. Biomed. Opt. 16(2) 021111 doi: 10.1117/1.3533314
Published in: Journal of Biomedical Optics Volume 16, Issue 2
Show Author Affiliations
Takeo Minamikawa, Osaka Univ. (Japan)
Tsutomu Araki, Osaka Univ. (Japan)
Mamoru Hashimoto, Osaka Univ. (Japan)
Hirohiko Niioka, Osaka Univ. (Japan)

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