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Journal of Biomedical Optics

Optical parametric oscillator-based light source for coherent Raman scattering microscopy: practical overview
Author(s): Sophie Brustlein; Patrick Ferrand; Nico Walther; Sophie Brasselet; Hervé Rigneault; Cyrille Billaudeau; Didier D. Marguet
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Paper Abstract

We present the assets and constraints of using optical parametric oscillators (OPOs) to perform point scanning nonlinear microscopy and spectroscopy with special emphasis on coherent Raman spectroscopy. The difterent possible configurations starting with one OPO and two OPOs are described in detail and with comments that are intended to be practically useful for the user. Explicit examples on test samples such as nonlinear organic crystal, polystyrene beads, and fresh mouse tissues are given. Special emphasis is given to background-free coherent Raman anti-Stokes scattering (CARS) imaging, including CARS hyperspectral imaging in a fully automated mode with commercial OPOs.

Paper Details

Date Published: 1 February 2011
PDF: 10 pages
J. Biomed. Opt. 16(2) 021106 doi: 10.1117/1.3533311
Published in: Journal of Biomedical Optics Volume 16, Issue 2
Show Author Affiliations
Sophie Brustlein, Institut Fresnel (France)
Patrick Ferrand, Institut Fresnel (France)
Nico Walther, Institut Fresnel (France)
Sophie Brasselet, Institut Fresnel (France)
Hervé Rigneault, Institut Fresnel (France)
Cyrille Billaudeau, Ctr. National de la Recherche Scientifique (France)
Didier D. Marguet, Ctr. National de la Recherche Scientifique (France)


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