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Journal of Biomedical Optics

Nonlinear microscopy, infrared, and Raman microspectroscopy for brain tumor analysis
Author(s): Tobias Meyer; Norbert Bergner; Christoph Krafft; Denis Akimov; Benjamin Dietzek; Jürgen Popp; Christiane Bielecki; Bernd F. M. Romeike; Rupert Reichart; Rolf Kalff
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Paper Abstract

Contemporary brain tumor research focuses on two challenges: First, tumor typing and grading by analyzing excised tissue is of utmost importance for choosing a therapy. Second, for prognostication the tumor has to be removed as completely as possible. Nowadays, histopathology of excised tissue using haematoxylin-eosine staining is the gold standard for the definitive diagnosis of surgical pathology specimens. However, it is neither applicable in vivo, nor does it allow for precise tumor typing in those cases when only nonrepresentative specimens are procured. Infrared and Raman spectroscopy allow for very precise cancer analysis due to their molecular specificity, while nonlinear microscopy is a suitable tool for rapid imaging of large tissue sections. Here, unstained samples from the brain of a domestic pig have been investigated by a multimodal nonlinear imaging approach combining coherent anti-Stokes Raman scattering, second harmonic generation, and two photon excited fluorescence microscopy. Furthermore, a brain tumor specimen was additionally analyzed by linear Raman and Fourier transform infrared imaging for a detailed assessment of the tissue types that is required for classification and to validate the multimodal imaging approach. Hence label-free vibrational microspectroscopic imaging is a promising tool for fast and precise in vivo diagnostics of brain tumors.

Paper Details

Date Published: 1 February 2011
PDF: 10 pages
J. Biomed. Opt. 16(2) 021113 doi: 10.1117/1.3533268
Published in: Journal of Biomedical Optics Volume 16, Issue 2
Show Author Affiliations
Tobias Meyer, Institut für Photonische Technologien e.V. (Germany)
Norbert Bergner, Institut für Photonische Technologien e.V. (Germany)
Christoph Krafft, Institut für Photonische Technologien e.V. (Germany)
Denis Akimov, Institut für Photonische Technologien e.V. (Germany)
Benjamin Dietzek, Institut für Photonische Technologien e.V. (Germany)
Jürgen Popp, Institut für Photonische Technologien e.V. (Germany)
Christiane Bielecki, Univ. Medical Ctr. Jena (Germany)
Bernd F. M. Romeike, Univ. Medical Ctr. Jena (Germany)
Rupert Reichart, Univ. Medical Ctr. Jena (Germany)
Rolf Kalff, Univ. Medical Ctr. Jena (Germany)


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