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Journal of Biomedical Optics • Open Access

Label-free imaging of Drosophila in vivo by coherent anti-Stokes Raman scattering and two-photon excitation autofluorescence microscopy
Author(s): Cheng-Hao Chien; Wei-Wen Chen; June-Tai Wu; Ta-Chau Chang

Paper Abstract

Drosophila is one of the most valuable model organisms for studying genetics and developmental biology. The fat body in Drosophila, which is analogous to the liver and adipose tissue in human, stores lipids that act as an energy source during its development. At the early stages of metamorphosis, the fat body remodeling occurs involving the dissociation of the fat body into individual fat cells. Here we introduce a combination of coherent anti-Stokes Raman scattering (CARS) and two-photon excitation autofluorescence (TPE-F) microscopy to achieve label-free imaging of Drosophila in vivo at larval and pupal stages. The strong CARS signal from lipids allows direct imaging of the larval fat body and pupal fat cells. In addition, the use of TPE-F microscopy allows the observation of other internal organs in the larva and autofluorescent globules in fat cells. During the dissociation of the fat body, the findings of the degradation of lipid droplets and an increase in autofluorescent globules indicate the consumption of lipids and the recruitment of proteins in fat cells. Through in vivo imaging and direct monitoring, CARS microscopy may help elucidate how metamorphosis is regulated and study the lipid metabolism in Drosophila.

Paper Details

Date Published: 1 January 2011
PDF: 7 pages
J. Biomed. Opt. 16(1) 016012 doi: 10.1117/1.3528642
Published in: Journal of Biomedical Optics Volume 16, Issue 1
Show Author Affiliations
Cheng-Hao Chien, National Yang-Ming Univ. (Taiwan)
Wei-Wen Chen, National Yang-Ming Univ. (Taiwan)
June-Tai Wu, National Taiwan Univ. (Taiwan)
Ta-Chau Chang, National Yang-Ming Univ. (Taiwan)


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