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Journal of Biomedical Optics • Open Access

Optoelectrophysiological stimulation of the human eye using fundus-controlled silent substitution technique
Author(s): Sascha Klee; Dietmar Link; Patrick Bessler; Jens Haueisen

Paper Abstract

We design, characterize, and apply a novel optoelectrophysiological setup for a fundus-controlled silent substitution technique that accounts for interindividual variability in retina morphology and simultaneously monitors the stimulation site under investigation. We connect a digital color liquid crystal on silicon projector, an electron-multiplying imager, and a light-emitting diode to a fundus camera. The temporal and spatial characterization reveal a maximal contrast loss of 7% for the highest stimulation frequency (30 Hz) and maximum cutoff spatial frequencies of ∼120 cycles/deg. Two silent substitution flash sequences are applied to modulate selective activity in the short-wavelength-sensitive cone (S-cone) and combined long- and middle-wavelength-sensitive cone (LM-cone) pathways. Simultaneously, the visual evoked potentials are recorded. The data are compared to the grand average responses from a previous study that employed standard computer-screen presentation and showed very good latency matches. All the volunteers in the present examination exhibit differences between the S-cone and LM-cone evoked potentials (parameters mean values: peak-to-peak amplitude, N1 latency, and P1 latency for S-cone/LM-cone responses: 8 μV/15 μV, 113 ms/89 ms, 170 ms/143 ms). We demonstrate that the developed optoelectrophysiological setup simultaneously provides imaging, functional stimulation, and electrophysiological investigation of the retina.

Paper Details

Date Published: 1 January 2011
PDF: 10 pages
J. Biomed. Opt. 16(1) 015002 doi: 10.1117/1.3528616
Published in: Journal of Biomedical Optics Volume 16, Issue 1
Show Author Affiliations
Sascha Klee, Technische Univ. Ilmenau (Germany)
Dietmar Link, Technische Univ. Ilmenau (Germany)
Patrick Bessler, Technische Univ. Ilmenau (Germany)
Jens Haueisen, Technische Univ. Ilmenau (Germany)

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