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Journal of Biomedical Optics

Two-dimensional singlet oxygen imaging with its near-infrared luminescence during photosensitization
Author(s): Bolin Hu; Nan Zeng; Zhiyi Liu; Yanhong Ji; Weidong Xie; Qing Peng; Yong Zhou; Yonghong He; Hui Ma
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Paper Abstract

Photodynamic therapy is a promising cancer treatment that involves activation of photosensitizer by visible light to create singlet oxygen. This highly reactive oxygen species is believed to induce cell death and tissue destruction in PDT. Our approach used a near-infrared area CCD with high quantum efficiency to detect singlet oxygen by its 1270-nm luminescence. Two-dimensional singlet oxygen images with its near-infrared luminescence during photosensitization could be obtained with a CCD integration time of 1 s, without scanning. Thus this system can produce singlet oxygen luminescence images faster and achieve more accurate measurements in comparison to raster-scanning methods. The experimental data show a linear relationship between the singlet oxygen luminescence intensity and sample concentration. This method provides a detection sensitivity of 0.0181 μg/ml (benzoporphyrin derivative monoacid ring A dissolved in ethanol) and a spatial resolution better than 50 μm. A pilot study was conducted on a total of six female Kunming mice. The results from this study demonstrate the system's potential for in vivo measurements. Further experiments were carried out on two tumor-bearing nude mice. Singlet oxygen luminescence images were acquired from the tumor-bearing nude mouse with intravenous injection of BPD-MA, and the experimental results showed real-time singlet oxygen signal depletion as a function of the light exposure.

Paper Details

Date Published: 1 January 2011
PDF: 7 pages
J. Biomed. Opt. 16(1) 016003 doi: 10.1117/1.3528593
Published in: Journal of Biomedical Optics Volume 16, Issue 1
Show Author Affiliations
Bolin Hu, Tsinghua Univ. (China)
Nan Zeng, Tsinghua Univ. (China)
Zhiyi Liu, Tsinghua Univ. (China)
Yanhong Ji, South China Normal Univ. (China)
Weidong Xie, Tsinghua Univ. (China)
Qing Peng, Shanghai Jiao Tong Univ. School of Medicine (China)
Yong Zhou, Tsinghua Univ. (China)
Yonghong He, Tsinghua Univ. (China)
Hui Ma, Tsinghua Univ. (China)

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