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Journal of Biomedical Optics • Open Access

Tracer-cocktail injections for combined pre- and intraoperative multimodal imaging of lymph nodes in a spontaneous mouse prostate tumor model
Author(s): Anne C. van Leeuwen; Tessa Buckle; Gavin Bendle; Lenka Vermeeren; Renato V. Olmos; Henk G. van de Poel; Fijs W. B. van Leeuwen

Paper Abstract

To improve surgical guidance toward prostate draining lymph nodes, we investigate the potential of intraoperative fluorescence imaging and combined pre- and intraoperative multimodality imaging approaches. Transgenic adenocarcinoma mouse prostate mice with spontaneous prostate tumors are injected intratumorally with: 1. a cocktail of patent blue (Pb) and indocyanine green (ICG); 2. a cocktail of albumin radiocolloids (99mTc-NanoColl), Pb, and ICG; or 3. a cocktail of radiolabeled albumin (99mTc-Vasculosis), Pb, and ICG. The distribution of these imaging agents over the lymph nodes (LNs) are studied at different time points after injection. We find that at 60-min postinjection, ICG significantly improves the detection of the LNs compared to Pb, 53 versus 7%, respectively. Moreover, a cocktail of ICG and 99mTc-NanoColl improves the fluorescent detection rate to 86%, equalling that of the clinically applied 99mTc-NanoColl. A similar overlap is observed in our initial clinical pilot data. Fluorescent detection of the LNs using a ICG with 99mTc-Vasculosis gives similar results as "free" ICG (58%; 60 min). A 99mTc-NanoColl, Pb, and cocktail ICG enriches the standard 99mTc-NanoColl approach by adding optical detection of the sentinel lymph nodes. Furthermore, this approach improves fluorescent-based guidance and enables both accurate surgical planning and intraoperative detection, based on a single injection.

Paper Details

Date Published: 1 January 2011
PDF: 11 pages
J. Biomed. Opt. 16(1) 016004 doi: 10.1117/1.3528027
Published in: Journal of Biomedical Optics Volume 16, Issue 1
Show Author Affiliations
Anne C. van Leeuwen, Antoni van Leeuwenhoek Hospital (Netherlands)
Tessa Buckle, Antoni van Leeuwenhoek Hospital (Netherlands)
Gavin Bendle, Antoni van Leeuwenhoek Hospital (Netherlands)
Lenka Vermeeren, Antoni van Leeuwenhoek Hospital (Netherlands)
Renato V. Olmos, Antoni van Leeuwenhoek Hospital (Netherlands)
Henk G. van de Poel, Antoni van Leeuwenhoek Hospital (Netherlands)
Fijs W. B. van Leeuwen, Antoni van Leeuwenhoek Hospital (Netherlands)


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