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Journal of Biomedical Optics • Open Access

Dual-trap Raman tweezers for probing dynamics and heterogeneity of interacting microbial cells
Author(s): Yan Li; Guiwen Wang; Hui-lu Yao; Junxian Liu; Yong-qing Li

Paper Abstract

We report on development of dual-trap Raman tweezers for monitoring cellular dynamics and heterogeneity of interacting living cells suspended in a liquid medium. Dual-beam optical tweezers were combined with Raman spectroscopy, which allows capturing two cells that are in direct contact or closely separated by a few micrometers and simultaneously acquiring their Raman spectra with an imaging CCD spectrograph. As a demonstration, we recorded time-lapse Raman spectra of budding yeast cells held in dual traps for over 40 min to monitor the dynamic growth in a nutrient medium. We also monitored two germinating Bacillus spores after the initiation with L-alanine and observed their heterogeneity in the release of CaDPA under identical microenvironment.

Paper Details

Date Published: 1 November 2010
PDF: 7 pages
J. Biomed. Opt. 15(6) 067008 doi: 10.1117/1.3526357
Published in: Journal of Biomedical Optics Volume 15, Issue 6
Show Author Affiliations
Yan Li, Guangxi Academy of Sciences (China)
Guiwen Wang, Guangxi Academy of Sciences (China)
Hui-lu Yao, Guangxi Academy of Sciences (China)
Junxian Liu, Guangxi Normal Univ. (China)
Yong-qing Li, East Carolina Univ. (United States)

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