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Journal of Biomedical Optics

Nanoscale nuclear architecture for cancer diagnosis beyond pathology via spatial-domain low-coherence quantitative phase microscopy
Author(s): Pin Wang; Rajan K. Bista; Walid E. Khalbuss; Wei Qiu; Shikhar Uttam; Kevin D. Staton; Lin Zhang; Teresa A. Brentnall; Randall E. Brand; Yang Liu
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Paper Abstract

Definitive diagnosis of malignancy is often challenging due to limited availability of human cell or tissue samples and morphological similarity with certain benign conditions. Our recently developed novel technology-spatial-domain low-coherence quantitative phase microscopy (SL-QPM)-overcomes the technical difficulties and enables us to obtain quantitative information about cell nuclear architectural characteristics with nanoscale sensitivity. We explore its ability to improve the identification of malignancy, especially in cytopathologically non-cancerous-appearing cells. We perform proof-of-concept experiments with an animal model of colorectal carcinogenesis-APCMin mouse model and human cytology specimens of colorectal cancer. We show the ability of in situ nanoscale nuclear architectural characteristics in identifying cancerous cells, especially in those labeled as "indeterminate or normal" by expert cytopathologists. Our approach is based on the quantitative analysis of the cell nucleus on the original cytology slides without additional processing, which can be readily applied in a conventional clinical setting. Our simple and practical optical microscopy technique may lead to the development of novel methods for early detection of cancer.

Paper Details

Date Published: 1 November 2010
PDF: 8 pages
J. Biomed. Opt. 15(6) 066028 doi: 10.1117/1.3523618
Published in: Journal of Biomedical Optics Volume 15, Issue 6
Show Author Affiliations
Pin Wang, Univ. of Pittsburgh (United States)
Rajan K. Bista, Univ. of Pittsburgh (United States)
Walid E. Khalbuss, Univ. of Pittsburgh Medical Ctr. (United States)
Wei Qiu, Univ. of Pittsburgh Medical Ctr. (United States)
Shikhar Uttam, Univ. of Pittsburgh (United States)
Kevin D. Staton, Univ. of Pittsburgh (United States)
Lin Zhang, Univ. of Pittsburgh (United States)
Teresa A. Brentnall, Univ. of Washington (United States)
Randall E. Brand, Univ. of Pittsburgh (United States)
Yang Liu, Univ. of Pittsburgh (United States)

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