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Journal of Biomedical Optics • Open Access

Quantification of in vivo fluorescence decoupled from the effects of tissue optical properties using fiber-optic spectroscopy measurements
Author(s): Anthony Kim; Mamta Khurana; Yumi Moriyama; Brian C. Wilson

Paper Abstract

We present a method for tissue fluorescence quantification in situ using a handheld fiber optic probe that measures both the fluorescence and diffuse reflectance spectra. A simplified method to decouple the fluorescence spectrum from distorting effects of the tissue optical absorption and scattering is developed, with the objective of accurately quantifying the fluorescence in absolute units. The primary motivation is measurement of 5-aminolevulinic acid-induced protoporphyrin IX (ALA-PpIX) concentration in tissue during fluorescence-guided resection of malignant brain tumors. This technique is validated in phantoms and ex vivo mouse tissues, and tested in vivo in a rabbit brain tumor model using ALA-PpIX fluorescence contrast.

Paper Details

Date Published: 1 November 2010
PDF: 12 pages
J. Biomed. Opt. 15(6) 067006 doi: 10.1117/1.3523616
Published in: Journal of Biomedical Optics Volume 15, Issue 6
Show Author Affiliations
Anthony Kim, Ontario Cancer Institute (Canada)
Mamta Khurana, Ontario Cancer Institute (Central African Republic)
Yumi Moriyama, Ontario Cancer Institute (Canada)
Brian C. Wilson, Ontario Cancer Institute (Canada)


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