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Journal of Biomedical Optics

Evaluation of high-performance liquid chromatography laser-induced fluorescence for serum protein profiling for early diagnosis of oral cancer
Author(s): Ajeetkumar Patil; Vijendra Prabhu; K. S. Choudhari; V. K. Unnikrishnan; Sajan D. George; Ravikiran Ongole; Keerthilatha M. Pai; Jayarama K. Shetty; Sujatha Bhat; Vasedevan B. Kartha; Santhosh Chidangil
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Paper Abstract

The present work deals with the evaluation of a high-performance liquid chromatography laser-induced fluorescence (HPLC-LIF) technique developed in our laboratory for early detection of oral cancer from protein profiles of body fluids. The results show that protein profiles of serum samples from a given class of samples, say, normal, premalignant, or malignant, are statistically very close to each other, while profiles of members of any class are significantly different from other classes. The performance of the technique is evaluated by the use of sensitivity and specificity pairs, receiver operating characteristic (ROC) analysis, and Youden's Index. The technique uses protein profile differences in serum samples, registered by the HPLC-LIF technique. The study is carried out using serum samples from volunteers diagnosed as normal or premalignant clinically, and as malignant by histopathology. The specificities and sensitivities of the HPLC-LIF method at an ideal threshold (M-distance = 2) for normal, malignant, and premalignant classes are 100, 69.5, and 61.5%, and 86.5, 87.5, and 87.5% respectively.

Paper Details

Date Published: 1 November 2010
PDF: 9 pages
J. Biomed. Opt. 15(6) 067007 doi: 10.1117/1.3523372
Published in: Journal of Biomedical Optics Volume 15, Issue 6
Show Author Affiliations
Ajeetkumar Patil, Manipal Univ. (India)
Vijendra Prabhu, Manipal Univ. (India)
K. S. Choudhari, Manipal Univ. (India)
V. K. Unnikrishnan, Manipal Univ. (India)
Sajan D. George, Technische Univ. Darmstadt (Germany)
Ravikiran Ongole, Manipal Univ. (India)
Keerthilatha M. Pai, Manipal Univ. (India)
Jayarama K. Shetty, Manipal Univ. (India)
Sujatha Bhat, Manipal Univ. (India)
Vasedevan B. Kartha, 21 Udayagiri (India)
Santhosh Chidangil, Manipal Univ. (India)


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