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Journal of Biomedical Optics • Open Access

Topographic mapping of subsurface fluorescent structures in tissue using multiwavelength excitation
Author(s): Anthony Kim; Mathieu Roy; Farhan N. Dadani; Brian C. Wilson

Paper Abstract

Different colors of visible light penetrate to varying depths in tissue due to the wavelength dependence of tissue optical absorption and elastic scattering. We exploit this to map the contour of the closest surface of a buried fluorescent object. This uses a novel algorithm based on the diffusion theory description of light propagation in tissue at each excitation wavelength to derive metrics that define the depth of the top surface of the object. The algorithm was validated using a tissue-simulating phantom. It was then demonstrated in vivo by subsurface brain tumor topography in a rodent model, using the fluorescence signal from protoporphyrin IX that is preferentially synthesized within malignant cells following systemic application of aminolevulinic acid. Comparisons to histomorphometry in the brain post mortem show the spatial accuracy of the technique. This method has potential for fluorescence image-guided tumor surgery, as well as other biomedical and nonbiological applications in subsurface sensing.

Paper Details

Date Published: 1 November 2010
PDF: 7 pages
J. Biomed. Opt. 15(6) 066026 doi: 10.1117/1.3523369
Published in: Journal of Biomedical Optics Volume 15, Issue 6
Show Author Affiliations
Anthony Kim, Ontario Cancer Institute (Canada)
Mathieu Roy, Ontario Cancer Institute (Canada)
Farhan N. Dadani, Ontario Cancer Institute (Canada)
Brian C. Wilson, Ontario Cancer Institute (Canada)

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