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Journal of Biomedical Optics • Open Access

Fiber-optic nonlinear endomicroscopy with focus scanning by using shape memory alloy actuation
Author(s): Yicong Wu; Yuying Zhang; Jiefeng Xi; Ming-Jun Li; Xingde Li

Paper Abstract

A miniature fiber optic endomicroscope with built-in dynamic focus scanning capability is developed for the first time for 3-D two-photon fluorescence (TPF) imaging of biological samples. Fast 2-D lateral beam scanning is realized by resonantly vibrating a double-clad fiber cantilever with a tubular piezoactuator. Slow axial scanning is achieved by moving the distal end of the imaging probe with an extremely compact electrically driven shape memory alloy (SMA). The 10-mm-long SMA allows 150-μm contractions with a driving voltage varying only from 50 to 100 mV. The response of the SMA contraction with the applied voltage is nonlinear, but repeatable and can be accurately calibrated. Depth-resolved imaging of acriflavine-stained biological tissues and unstained white paper with the endomicroscope is performed, and the results demonstrate the feasibility of 3-D nonlinear optical imaging with the SMA-based scanning fiber-optic endomicroscope.

Paper Details

Date Published: 1 November 2010
PDF: 3 pages
J. Biomed. Opt. 15(6) 060506 doi: 10.1117/1.3523234
Published in: Journal of Biomedical Optics Volume 15, Issue 6
Show Author Affiliations
Yicong Wu, The Johns Hopkins Univ. (United States)
Yuying Zhang, The Johns Hopkins Univ. (United States)
Jiefeng Xi, The Johns Hopkins Univ. (United States)
Ming-Jun Li, Corning Incorporated (United States)
Xingde Li, The Johns Hopkins Univ. (United States)

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