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Journal of Biomedical Optics

Label-free imaging of biomolecules in food products using stimulated Raman microscopy
Author(s): Maarten B. J. Roeffaers; Xu Zhang; Christian W. Freudiger; Brian G. Saar; X. Sunney Xie; Marjolein van Ruijven; Gerard van Dalen; Chunhong Xiao
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Paper Abstract

The development of methods that allow microscale studies of complex biomaterials based on their molecular composition is of great interest to a wide range of research fields. We show that stimulated Raman scattering (SRS) microscopy is an excellent analytical tool to study distributions of different biomolecules in multiphasic systems. SRS combines the label-free molecular specificity of vibrational spectroscopy with an enhanced sensitivity due to coherent excitation of molecular vibrations. Compared to previous imaging studies using coherent anti-Stokes Raman scattering microscopy, the main advantage of SRS microscopy is the absence of the unwanted nonresonant background, which translates into a superior sensitivity and undistorted vibrational spectra. We compare spectra of complex materials obtained with stimulated Raman scattering and spontaneous Raman scattering in the crowded fingerprint region. We find that, as expected, there is excellent correspondence and that the SRS spectra are free from interference from background fluorescence. In addition, we show high-resolution imaging of the distributions of selected biomolecules, such as lipids and proteins, in food products with SRS microscopy.

Paper Details

Date Published: 1 February 2011
PDF: 6 pages
J. Biomed. Opt. 16(2) 021118 doi: 10.1117/1.3516591
Published in: Journal of Biomedical Optics Volume 16, Issue 2
Show Author Affiliations
Maarten B. J. Roeffaers, Harvard Univ. (United States)
Xu Zhang, Harvard Univ. (United States)
Christian W. Freudiger, Harvard Univ. (United States)
Brian G. Saar, Harvard Univ. (United States)
X. Sunney Xie, Harvard Univ. (United States)
Marjolein van Ruijven, Unilever Research & Development (United States)
Gerard van Dalen, Unilever Research & Development (United States)
Chunhong Xiao, Unilever Research & Development (United States)

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