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Journal of Biomedical Optics • Open Access

Optical imaging of reporter gene expression using a positron-emission-tomography probe
Author(s): Hongguang Liu; Gang Ren; Shuanglong Liu; Xiaofen Zhang; Luxi Chen; Peizhen Han; Zhen Cheng

Paper Abstract

Reporter gene/reporter probe technology is one of the most important techniques in molecular imaging. Lately, many reporter gene/reporter probe systems have been coupled to different imaging modalities such as positron emission tomography (PET) and optical imaging (OI). It has been recently found that OI techniques could be used to monitor radioactive tracers in vitro and in living subjects. In this study, we further demonstrate that a reporter gene/nuclear reporter probe system [herpes simplex virus type-1 thymidine kinase (HSV1-tk) and 9-(4-18F-fluoro-3-[hydroxymethyl] butyl) guanine ([18F]FHBG)] could be successfully imaged by OI in vitro and in vivo. OI with radioactive reporter probes will facilitate and broaden the applications of reporter gene/reporter probe techniques in medical research.

Paper Details

Date Published: 1 November 2010
PDF: 3 pages
J. Biomed. Opt. 15(6) 060505 doi: 10.1117/1.3514659
Published in: Journal of Biomedical Optics Volume 15, Issue 6
Show Author Affiliations
Hongguang Liu, Stanford Univ. (United States)
Gang Ren, Stanford Univ. (United States)
Shuanglong Liu, Stanford Univ. (United States)
Xiaofen Zhang, Stanford Univ. (United States)
Luxi Chen, Stanford Univ. (United States)
Peizhen Han, Tsinghua Univ. (China)
Zhen Cheng, Stanford Univ. (United States)

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