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Journal of Biomedical Optics

Infrared spectroscopy reveals both qualitative and quantitative differences in equine subchondral bone during maturation
Author(s): Yevgeniya Kobrina; Hanna Isaksson; Miikka Sinisaari; Lassi L. Rieppo; Pieter A. Brama; Rene van Weeren; Heikki J. Helminen; Jukka S. Jurvelin; Simo Saarakkala
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Paper Abstract

The collagen phase in bone is known to undergo major changes during growth and maturation. The objective of this study is to clarify whether Fourier transform infrared (FTIR) microspectroscopy, coupled with cluster analysis, can detect quantitative and qualitative changes in the collagen matrix of subchondral bone in horses during maturation and growth. Equine subchondral bone samples (n = 29) from the proximal joint surface of the first phalanx are prepared from two sites subjected to different loading conditions. Three age groups are studied: newborn (0 days old), immature (5 to 11 months old), and adult (6 to 10 years old) horses. Spatial collagen content and collagen cross-link ratio are quantified from the spectra. Additionally, normalized second derivative spectra of samples are clustered using the k-means clustering algorithm. In quantitative analysis, collagen content in the subchondral bone increases rapidly between the newborn and immature horses. The collagen cross-link ratio increases significantly with age. In qualitative analysis, clustering is able to separate newborn and adult samples into two different groups. The immature samples display some nonhomogeneity. In conclusion, this is the first study showing that FTIR spectral imaging combined with clustering techniques can detect quantitative and qualitative changes in the collagen matrix of subchondral bone during growth and maturation.

Paper Details

Date Published: 1 November 2010
PDF: 9 pages
J. Biomed. Opt. 15(6) 067003 doi: 10.1117/1.3512177
Published in: Journal of Biomedical Optics Volume 15, Issue 6
Show Author Affiliations
Yevgeniya Kobrina, Univ. of Eastern Finland (Finland)
Hanna Isaksson, Univ. of Eastern Finland (Finland)
Miikka Sinisaari, Univ. of Eastern Finland (Finland)
Lassi L. Rieppo, Univ. of Eastern Finland (Finland)
Pieter A. Brama, Univ. College Dublin (Ireland)
Rene van Weeren, Utrecht Univ. (Netherlands)
Heikki J. Helminen, Univ. of Eastern Finland (Finland)
Jukka S. Jurvelin, Univ. of Eastern Finland (Finland)
Simo Saarakkala, Univ. of Eastern Finland (Finland)

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