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Journal of Biomedical Optics • Open Access

Selective suppression of two-photon fluorescence in laser scanning microscopy by ultrafast pulse-train excitation

Paper Abstract

Selective excitation of a particular fluorophore in the presence of others demands clever design of the optical field interacting with the molecules. We describe the use of 20- to 50-GHz pulse-train excitation leading to two-photon absorption, followed by successive one-photon stimulated emission as a potential technique in the context of controlling two-photon molecular fluorescence, with applications in microscopy.

Paper Details

Date Published: 1 November 2010
PDF: 3 pages
J. Biomed. Opt. 15(6) 060502 doi: 10.1117/1.3509383
Published in: Journal of Biomedical Optics Volume 15, Issue 6
Show Author Affiliations
Arijit Kumar De, Indian Institute of Technology Kanpur (India)
Debjit Roy, Indian Institute of Technology Kanpur (India)
Debabrata Goswami, Indian Institute of Technology Kanpur (India)

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