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Journal of Biomedical Optics • Open Access

Analysis and three-dimensional visualization of collagen in artificial scaffolds using nonlinear microscopy techniques
Author(s): Eva Filová; Zuzana Burdíková; Michala Rampichová; Paolo Bianchini; Martin Capek; Eva Košt’áková; Evzen Amler; Lucie Kubínová

Paper Abstract

Extracellularly distributed collagen and chondrocytes seeded in gelatine and poly--caprolactone scaffolds are visualized by two-photon excitation microscopy (TPEM) and second-harmonic generation (SHG) imaging in both forward and backward nondescanned modes. Joint application of TPEM and SHG imaging in combination with stereological measurements of collagen enables us not only to take high-resolution 3-D images, but also to quantitatively analyze the collagen volume and a spatial arrangement of cell-collagen-scaffold systems, which was previously impossible. This novel approach represents a powerful tool for the analysis of collagen-containing scaffolds with applications in cartilage tissue engineering.

Paper Details

PDF: 7 pages
J. Biomed. Opt. 15(6) 066011 doi: 10.1117/1.3509112
Published in: Journal of Biomedical Optics Volume 15, Issue 6, November 2010
Show Author Affiliations
Eva Filová, The Czech Academy of Sciences (Czech Republic)
Zuzana Burdíková, The Czech Academy of Sciences (Czech Republic)
Michala Rampichová, The Czech Academy of Sciences (Czech Republic)
Paolo Bianchini, Istituto Italiano di Tecnologia (Italy)
Martin Capek, The Czech Academy of Sciences (Czech Republic)
Eva Košt’áková, Technical Univ. of Liberec (Czech Republic)
Evzen Amler, The Czech Academy of Sciences (Czech Republic)
Lucie Kubínová, The Czech Academy of Sciences (Czech Republic)


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