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Journal of Biomedical Optics • Open Access

Localization capability and limitation of electron-multiplying charge-coupled, scientific complementary metal-oxide semiconductor, and charge-coupled devices for superresolution imaging
Author(s): Tingwei Quan; Shaoqun Zeng; Zhenli Huang

Paper Abstract

Localization of a single fluorescent molecule is required in a number of superresolution imaging techniques for visualizing biological structures at cellular and subcellular levels. The localization capability and limitation of low-light detectors are critical for such a purpose. We present an updated evaluation on the performance of three typical low-light detectors, including a popular electron-multiplying CCD (EMCCD), a newly developed scientific CMOS (sCMOS), and a representative cooled CCD, for superresolution imaging. We find that under some experimental accessible conditions, the sCMOS camera shows a competitive and even better performance than the EMCCD camera, which has long been considered the detector of choice in the field of superresolution imaging.

Paper Details

Date Published: 1 November 2010
PDF: 6 pages
J. Biomed. Opt. 15(6) 066005 doi: 10.1117/1.3505017
Published in: Journal of Biomedical Optics Volume 15, Issue 6
Show Author Affiliations
Tingwei Quan, Huazhong Univ. of Science and Technology (China)
Shaoqun Zeng, Britton Chance Ctr. for Biomedical Photonics (China)
Zhenli Huang, Britton Chance Ctr. for Biomedical Photonics (China)

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