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Journal of Biomedical Optics

Spectral Doppler optical coherence tomography imaging of localized ischemic stroke in a mouse model
Author(s): Lingfeng Yu; Elaine Nguyen; Gangjun Liu; Bernard Choi; Zhongping Chen
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Paper Abstract

We report the use of spectral Doppler optical coherence tomography imaging (SDOCTI) for quantitative evaluation of dynamic blood circulation before and after a localized ischemic stroke in a mouse model. Rose Bengal photodynamic therapy (PDT) is used as a noninvasive means for inducing localized ischemia in cortical microvasculature of the mouse. Fast, repeated Doppler optical coherence tomography scans across vessels of interest are performed to record flow dynamic information with high temporal resolution. Doppler-angle-independent flow indices are used to quantify vascular conditions before and after the induced ischemia by the photocoagulation of PDT. The higher (or lower) flow resistive indices are associated with higher (or lower) resistance states that are confirmed by laser speckle flow index maps (of laser speckle imaging). Our in vivo experiments shows that SDOCTI can provide complementary quantified flow information that is an alternative to blood volume measurement, and can be used as a means for cortical microvasculature imaging well suited for small animal studies.

Paper Details

Date Published: 1 November 2010
PDF: 6 pages
J. Biomed. Opt. 15(6) 066006 doi: 10.1117/1.3505016
Published in: Journal of Biomedical Optics Volume 15, Issue 6
Show Author Affiliations
Lingfeng Yu, Beckman Laser Institute and Medical Clinic (United States)
Elaine Nguyen, Beckman Laser Institute and Medical Clinic (United States)
Gangjun Liu, Beckman Laser Institute and Medical Clinic (United States)
Bernard Choi, Beckman Laser Institute and Medical Clinic (United States)
Zhongping Chen, Beckman Laser Institute and Medical Clinic (United States)


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