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Journal of Biomedical Optics • Open Access

Identification of different bacterial species in biofilms using confocal Raman microscopy
Author(s): Brooke D. Beier; Robert G. Quivey; Andrew J. Berger

Paper Abstract

Confocal Raman microspectroscopy is used to discriminate between different species of bacteria grown in biofilms. Tests are performed using two bacterial species, Streptococcus sanguinis and Streptococcus mutans, which are major components of oral plaque and of particular interest due to their association with healthy and cariogenic plaque, respectively. Dehydrated biofilms of these species are studied as a simplified model of dental plaque. A prediction model based on principal component analysis and logistic regression is calibrated using pure biofilms of each species and validated on pure biofilms grown months later, achieving 96% accuracy in prospective classification. When biofilms of the two species are partially mixed together, Raman-based identifications are achieved within ~2 µm of the boundaries between species with 97% accuracy. This combination of spatial resolution and predication accuracy should be suitable for forming images of species distributions within intact two-species biofilms.

Paper Details

Date Published: 1 November 2010
PDF: 5 pages
J. Biomed. Opt. 15(6) 066001 doi: 10.1117/1.3505010
Published in: Journal of Biomedical Optics Volume 15, Issue 6
Show Author Affiliations
Brooke D. Beier, Univ. of Rochester (United States)
Robert G. Quivey, Univ. of Rochester Medical Ctr. (United States)
Andrew J. Berger, Univ. of Rochester (United States)


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