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Journal of Biomedical Optics

Light scattering and morphology of the lymphocyte as applied to flow cytometry for distinguishing healthy and infected individuals
Author(s): Gennady I. Ruban; Vladimir V. Berdnik; Dmitry V. Marinitch; Natalia V. Goncharova; Valery A. Loiko
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Paper Abstract

A simple optical model of single lymphocytes with smooth and nonsmooth surfaces has been developed for healthy and infected individuals. The model can be used for rapid (in the real-time scale) solution of the inverse light-scattering problem on the basis of optical data measured by label-free flow cytometry. Light scattering patterns have been calculated for the model developed. It has been shown that the smooth and nonsmooth cells can be resolved using the intensities of the sideward- and backward-scattered light. We have found by calculations and validated by the flow cytometer experiments that intensity distributions for the cells of lymphocyte populations can be used as a preliminary signatures of some virus infections. Potential biomedical applications of the findings for label-free flow cytometry detection of individuals infected with viruses of hepatitises B or C and some others viruses are presented.

Paper Details

Date Published: 1 September 2010
PDF: 9 pages
J. Biomed. Opt. 15(5) 057008 doi: 10.1117/1.3503404
Published in: Journal of Biomedical Optics Volume 15, Issue 5
Show Author Affiliations
Gennady I. Ruban, B.I. Stepanov Institute of Physics (Belarus)
Vladimir V. Berdnik, B.I. Stepanov Institute of Physics (Belarus)
Dmitry V. Marinitch, Ctr. of Hematology and Transfusiology (Belarus)
Natalia V. Goncharova, Ctr. of Hematology and Transfusiology (Belarus)
Valery A. Loiko, B.I. Stepanov Institute of Physics (Belarus)


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