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Journal of Biomedical Optics • Open Access

Pulse-modulated second harmonic imaging microscope quantitatively demonstrates marked increase of collagen in tumor after chemotherapy
Author(s): Anju M. Raja; Shuoyu Xu; Wanxin Sun; Jianbiao Zhou; Dean C. S. Tai; Chien-Shing Chen; Jagath C. Rajapakse; Peter T. C. So; Hanry Yu

Paper Abstract

Pulse-modulated second harmonic imaging microscopes (PM-SHIMs) exhibit improved signal-to-noise ratio (SNR) over conventional SHIMs on sensitive imaging and quantification of weak collagen signals inside tissues. We quantify the spatial distribution of sparse collagen inside a xenograft model of human acute myeloid leukemia (AML) tumor specimens treated with a new drug against receptor tyrosine kinase (ABT-869), and observe a significant increase in collagen area percentage, collagen fiber length, fiber width, and fiber number after chemotherapy. This finding reveals new insights into tumor responses to chemotherapy and suggests caution in developing new drugs and therapeutic regimens against cancers.

Paper Details

Date Published: 1 September 2010
PDF: 11 pages
J. Biomed. Opt. 15(5) 056016 doi: 10.1117/1.3497565
Published in: Journal of Biomedical Optics Volume 15, Issue 5
Show Author Affiliations
Anju M. Raja, Institute of Bioengineering and Nanotechnology (Singapore)
Shuoyu Xu, Institute of Bioengineering and Nanotechnology (Singapore)
Wanxin Sun, Institute of Bioengineering and Nanotechnology (Singapore)
Jianbiao Zhou, National Univ. Health System (Singapore)
Dean C. S. Tai, Institute of Bioengineering and Nanotechnology (Singapore)
Chien-Shing Chen, National Univ. Health System (Singapore)
Jagath C. Rajapakse, Singapore-MIT Alliance (Singapore)
Peter T. C. So, Singapore-MIT Alliance (United States)
Hanry Yu, Institute of Bioengineering and Nanotechnology (Singapore)


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