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Journal of Biomedical Optics

Hemoglobin degradation in human erythrocytes with long-duration near-infrared laser exposure in Raman optical tweezers
Author(s): Raktim Dasgupta; Sunita Ahlawat; Ravi Shanker Verma; Abha Uppal; Pradeep Kumar Gupta
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Paper Abstract

Near-infrared laser (785-nm)-excited Raman spectra from a red blood cell, optically trapped using the same laser beam, show significant changes as a function of trapping duration even at trapping power level of a few milliwatts. These changes in the Raman spectra and the bright-field images of the trapped cell, which show a gradual accumulation of the cell mass at the trap focus, suggest photoinduced aggregation of intracellular heme. The possible role of photoinduced protein denaturation and hemichrome formation in the observed aggregation of heme is discussed.

Paper Details

Date Published: 1 September 2010
PDF: 11 pages
J. Biomed. Opt. 15(5) 055009 doi: 10.1117/1.3497048
Published in: Journal of Biomedical Optics Volume 15, Issue 5
Show Author Affiliations
Raktim Dasgupta, Raja Ramanna Ctr. for Advanced Technology (India)
Sunita Ahlawat, Raja Ramanna Ctr. for Advanced Technology (India)
Ravi Shanker Verma, Raja Ramanna Ctr. for Advanced Technology (India)
Abha Uppal, Raja Ramanna Ctr. for Advanced Technology (India)
Pradeep Kumar Gupta, Raja Ramanna Ctr. for Advanced Technology (India)


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