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Journal of Biomedical Optics

Visible light optical spectroscopy is sensitive to neovascularization in the dysplastic cervix
Author(s): Vivide Tuan-Chyan Chang; Sarah Bean; Peter S. Cartwright; Nirmala Ramanujam
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Paper Abstract

Neovascularization in cervical intraepithelial neoplasia (CIN) is studied because it is the precursor to the third most common female cancer worldwide. Diffuse reflectance from 450-600 nm was collected from 46 patients (76 sites) undergoing colposcopy at Duke University Medical Center. Total hemoglobin, derived using an inverse Monte Carlo model, significantly increased in CIN 2+ (N=12) versus CIN 1 (N=16) and normal tissues (N=48) combined with P<0.004. Immunohistochemistry using monoclonal anti-CD34 was used to quantify microvessel density to validate the increased hemoglobin content. Biopsies from 51 sites were stained, and up to three hot spots per slide were selected for microvessel quantification by two observers. Similar to the optical study results, microvessel density was significantly increased in CIN 2+ (N=16) versus CIN 1 (N=21) and normal tissue (N=14) combined with P<0.007. Total vessel density, however, was not significantly associated with dysplastic grade. Hence, our quantitative optical spectroscopy system is primarily sensitive to dysplastic neovascularization immediately beneath the basement membrane, with minimal confounding from vascularity inherent in the normal stromal environment. This tool could have potential for in vivo applications in screening for cervical cancer, prognostics, and monitoring of antiangiogenic effects in chemoprevention therapies.

Paper Details

Date Published: 1 September 2010
PDF: 9 pages
J. Biomed. Opt. 15(5) 057006 doi: 10.1117/1.3495730
Published in: Journal of Biomedical Optics Volume 15, Issue 5
Show Author Affiliations
Vivide Tuan-Chyan Chang, Duke Univ. (United States)
Sarah Bean, Duke Univ. (United States)
Peter S. Cartwright, Duke Univ. Medical Ctr. (United States)
Nirmala Ramanujam, Duke Univ. (United States)

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