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Journal of Biomedical Optics • Open Access

Enhancing precision in time-domain fluorescence lifetime imaging
Author(s): Ching-Wei Chang; Mary-Ann Mycek

Paper Abstract

In biological applications of fluorescence lifetime imaging, low signals from samples can be a challenge, causing poor lifetime precision. We demonstrate how optimal signal gating (a method applied to the temporal dimension of a lifetime image) and novel total variation denoising models (a method applied to the spatial dimension of a lifetime image) can be used in time-domain fluorescence lifetime imaging microscopy (FLIM) to improve lifetime precision. In time-gated FLIM, notable fourfold precision improvements were observed in a low-light example. This approach can be employed to improve FLIM data while minimizing sample light exposure and increasing imaging speed.

Paper Details

Date Published: 1 September 2010
PDF: 9 pages
J. Biomed. Opt. 15(5) 056013 doi: 10.1117/1.3494566
Published in: Journal of Biomedical Optics Volume 15, Issue 5
Show Author Affiliations
Ching-Wei Chang, Univ. of Michigan (United States)
Mary-Ann Mycek, Univ. of Michigan (United States)


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